Human Cyclophilin A/PPIA Recombinant Protein
Human Cyclophilin A/PPIA Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-165aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, Cyclophilin A, Cyclosporin A binding protein, Peptidyl prolyl cis trans isomerase A, PPIA, PPIA protein, PPIase A, Rotamase A, RotamaseA, T cell cyclophilin.
Gene Name
PPIA
UniProt
P62937
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Cell Cycle
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 650 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
20kDa (185aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 20mM NaCl, 0.5mM DTT, 10% glycerol
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