Human PP1 Catalytic Recombinant Protein
Human PP1 Catalytic Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-330aa. Application (s) : SDS-PAGE, Enzyme Activity.
Product Specifications
Synonyms
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A
Gene Name
PPP1CA
UniProt
P62136
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 80% by SDS-PAGE
Concentration
0.2mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH7.5 using 10mM of substrate.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
39.7 kDa (350aa) confirmed by MALDI-TOF
Contents
50mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol
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