E.coli mdh Recombinant Protein
E. coli mdh Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-312aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Malate dehydrogenase, ECK3225, JW3205
Gene Name
Mdh
UniProt
P61889
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Metabolism
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
34.9 kDa (336aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
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