E.coli Carbonic anhydrase 2 Recombinant Protein
E. coli Carbonic anhydrase 2 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-220aa. Application (s) : SDS-PAGE, Enzyme Activity.
Product Specifications
Synonyms
Carbonate dehydratase, CAN, ECK0125, JW0122, yadF
Gene Name
Can
UniProt
P61517
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
27.2 kDa (240aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
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