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E.coli Carbonic anhydrase 2 Recombinant Protein

E. coli Carbonic anhydrase 2 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-220aa. Application (s) : SDS-PAGE, Enzyme Activity.

Product Specifications

Synonyms

Carbonate dehydratase, CAN, ECK0125, JW0122, yadF

Gene Name

Can

UniProt

P61517

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Enzymes and Proteases

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

27.2 kDa (240aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

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