Mouse Pkm Recombinant Protein
Mouse Pkm Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-531aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Pyruvate kinase PKM isoform M2, Pyruvate kinase muscle isozyme, pyruvate kinase M1/2, Threonine-protein kinase PKM2, Tyrosine-protein kinase PKM2, Pk3, Pkm2, Pykm
Gene Name
Pkm
UniProt
P52480
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Cancer
Purification
> 85% by SDS-PAGE
Concentration
0.5mg/mL (determined by absorbance at 280nm)
Bioactivity
Specific activity is > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
60.2 kDa (554aa) Confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol
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