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Human Caspase-3 Recombinant Protein

Human Caspase-3 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 29-277aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

CASP3, CPP32, CPP32B, SCA-1, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1

Gene Name

CASP3

UniProt

P42574

Tag

His-Tag

Source

Baculovirus

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Neuroscience

Purification

> 90% by SDS-PAGE

Concentration

0.5mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 5,000 pmol/min/ug. One unit will liberate 1 pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

29.4 kDa (256aa) ns)

Contents

20mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol

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