Human Caspase-3 Recombinant Protein
Human Caspase-3 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 29-277aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
CASP3, CPP32, CPP32B, SCA-1, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1
Gene Name
CASP3
UniProt
P42574
Tag
His-Tag
Source
Baculovirus
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Neuroscience
Purification
> 90% by SDS-PAGE
Concentration
0.5mg/mL (determined by absorbance at 280nm)
Bioactivity
Specific activity is > 5,000 pmol/min/ug. One unit will liberate 1 pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
29.4 kDa (256aa) ns)
Contents
20mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol
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