Human Aldo-keto Reductase 1C3/AKR1C3 Recombinant Protein
Human Aldo-keto Reductase 1C3/AKR1C3 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-323aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Aldo-keto reductase family 1 member C3, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, Aldo-keto reductase family 1, member C3 17 beta HSD 5 (HSD17B5), AKR1 C3, Chlordecone reductase, 17 beta hydroxysteroid dehydrogenase type 5, DDH1, PGFS, Prostaglandin F synthase
Gene Name
AKR1C3
UniProt
P42330
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Redox Proteins
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
39 kDa (343aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
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