Human MDH1 Recombinant Protein
Human MDH1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-334aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Malate dehydrogenase cytoplasmic, MDH-s, MDHA, MOR2, Diiodophenylpyruvate reductase
Gene Name
MDH1
UniProt
P40925
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Cancer
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
37.4 kDa (342aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
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