Human PKLR Recombinant Protein
Human PKLR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 47-574aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK
Gene Name
PKLR
UniProt
P30613
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Cancer
Purification
> 90% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 80 units/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
59.2 kDa (549aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
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