Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein
Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 30-207aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Cyclophilin D, Cyclophilin F, CYP3, CYPD, hCyP3, Mitochondral, Peptidyl prolyl cis trans isomerase, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F Cyclophilin 3, PPIase, PPIF, Rotamase
Gene Name
PPIF
UniProt
P30405
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
21.1 kDa (199aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
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