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Human recombinant CD40L (CD40 ligand) protein, AF

Human CD40L (CD154) is one member of TNF family which is expressed on T cells. It binds the CD40, which is on the antigen-presenting cells (ex: B cells, macrophages and monocytes) Human CD40L is a 21.6 kDa cytokine with 103 amino acid residues which is a transmembrane glycoprotein. Human CD40L plays an important role in the adaptive immune system which induces the B cell maturation and activation.

Product Specifications

Synonyms

Soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM, sCD40 Ligand

Gene Name

CD40LG

UniProt

P29965

Reactivity

Human

Tag

His Tag (C-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>95% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <5 ng/mL.

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 16.99 kDa. The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PROTP29965-5

AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL with polyhistidine tag at the C-terminus
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