Mouse PDIA3 Recombinant Protein
Mouse PDIA3 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 25-505aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Protein disulfide isomerase associated 3, 58 kDa glucose-regulated protein, GRP58, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, Erp, PDI, PDI-Q2, Plca
Gene Name
Pdia3
UniProt
P27773
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Heat Shock Proteins
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is >10 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
56.8 kDa (505aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, and 10% glycerol
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