Renilla reniformis Luciferase Recombinant Protein
Renilla reniformis Luciferase Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-311aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Luciferase
UniProt
P27652
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is >1X10^9 light units/mg. One luciferase enzyme unit will produce one Relative Light Unit (RLU) at pH7.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
38.5 kDa (335aa) Confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
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