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Renilla reniformis Luciferase Recombinant Protein

Renilla reniformis Luciferase Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-311aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Luciferase

UniProt

P27652

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Enzymes and Proteases

Purification

> 95% by SDS-PAGE

Concentration

0.5mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is >1X10^9 light units/mg. One luciferase enzyme unit will produce one Relative Light Unit (RLU) at pH7.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

38.5 kDa (335aa) Confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

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