Mouse IL-1ra/IL-1F3 Recombinant Protein
Mouse IL-1ra/IL-1F3 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 27-178aa. Application (s) : SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RA
Gene Name
Il1rn
UniProt
P25085
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Bioactivity
Field of Research
Hormones and Cytokines
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/mL with Mouse IL-1 alpha.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
20 kDa (177aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
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