Human PTP1B Recombinant Protein
Human PTP1B Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-321aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Protein tyrosine phosphatase non-receptor type 1, PTPN1, PTP1B, PTP-1B, Protein Tyrosine Phosphatase 1B, EC 3.1.3.48, Protein-tyrosine phosphatase1B, Tyrosine-protein phosphatase non-receptor type 1, Protein tyrosine phosphatase non-receptor type 1, Protein Tyrosine Phosphatase Non Receptor Type-1
Gene Name
PTPN1
UniProt
P18031
Tag
Non-Tagged
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Signal Transduction
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
37.3 kDa (321aa)
Contents
25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA1mM DTT, 20% glycerol
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