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Mouse Enolase 1/Non-neural enolase Recombinant Protein

Mouse Enolase 1/Non-neural enolase Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-434aa. Application (s) : SDS-PAGE, Enzyme Activity.

Product Specifications

Synonyms

Alpha-Enolase, 0610008I15, AL022784, Eno-1, MBP-1

Gene Name

Eno1

UniProt

P17182

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Metabolism

Purification

> 90% by SDS-PAGE

Concentration

1mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37°C in a couple system with PK and LDH.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

49.5 kDa (457aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.1M NaCl

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