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Mouse L-lactate dehydrogenase B chain/Ldhb Recombinant Protein

Mouse L-lactate dehydrogenase B chain/Ldhb Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-334aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, LDH heart subunit

Gene Name

Ldhb

UniProt

P16125

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Cancer

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 150 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

39 kDa (357aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

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