Mouse L-lactate dehydrogenase B chain/Ldhb Recombinant Protein
Mouse L-lactate dehydrogenase B chain/Ldhb Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-334aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, LDH heart subunit
Gene Name
Ldhb
UniProt
P16125
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Cancer
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by absorbance at 280nm)
Bioactivity
Specific activity is > 150 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
39 kDa (357aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
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