Human recombinant HGF protein, AF

Hepatocyte Growth Factor (HGF) activates c-Met receptor's tyrosine kinase cascade, influencing cell growth and motility. Originating from mesenchymal cells, HGF is a multifunctional cytokine that plays a central role in angiogenesis, tumorigenesis, and tissue regeneration. Initially secreted as an inactive polypeptide, HGF undergoes cleavage to become an active heterodimeric molecule. The gene exhibits alternative splicing, resulting in diverse isoforms.

Product Specifications

Synonyms

Hepatocyte growth factor, Hepatopoietin-A, Scatter factor (SF), HPTA

Gene Name

HGF

UniProt

P14210

Reactivity

Human

Tag

His Tag (C-term)

Source

HEK293 cell

Applications

Cell Culture

Purification

>90% as determined by SDS-PAGE.

Endotoxin

<1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Testing in process

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 80.48 kDa. The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.