Human Enolase 3/Muscle-specific enolase Recombinant Protein
Human Enolase 3/Muscle-specific enolase Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-434aa. Application (s) : SDS-PAGE, Enzyme Activity.
Product Specifications
Synonyms
Beta-enolase, MSE
Gene Name
ENO3
UniProt
P13929
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Metabolism
Purification
> 95% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 5,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
49 kDa (454aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
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