E.coli uDP Recombinant Protein
E. coli uDP Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-253aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Uridine phosphorylase, UrdPase, Upase
Gene Name
Udp
UniProt
P12758
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
29.3 kDa (273aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
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