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E.coli uDP Recombinant Protein

E. coli uDP Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-253aa. Application (s) : Enzyme Activity, SDS-PAGE.

Product Specifications

Synonyms

Uridine phosphorylase, UrdPase, Upase

Gene Name

Udp

UniProt

P12758

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Enzymes and Proteases

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

29.3 kDa (273aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl

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