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Mouse IL-1 beta /IL-1F2 Recombinant Protein

Mouse IL-1 beta /IL-1F2 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 118-269aa. Application (s) : SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Interleukin-1 beta, IL-1 beta

Gene Name

Il1b

UniProt

P10749

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Bioactivity

Field of Research

Hormones and Cytokines

Purification

> 90% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤0.1 ng/mL.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

21 kDa (189aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

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