E.coli G6PD Recombinant Protein
E. coli G6PD Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-491aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Glucose-6-phosphate 1-dehydrogenase, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6PD1, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p.
Gene Name
Zwf
UniProt
P0AC53
Tag
Non-Tagged
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 50 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
55.7 kDa (491aa) confirmed by MALDI-TOF
Contents
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
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