E.coli TRXB Recombinant Protein
E. coli TRXB Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-321aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, FAD/NAD (P) -binding, TRXR
Gene Name
TrxB
UniProt
P0A9P4
Tag
Non-Tagged
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Redox Proteins
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
34.6 kDa (321aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
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