Human UCH-L1/PGP9.5 Recombinant Protein
Human UCH-L1/PGP9.5 Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-223aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Ubiquitin C-terminal hydrolase L1, Ubiquitin carboxyl-terminal esterase L1, Ubiquitin thiolesterase L1, Parkinson Disease 5, PARK5, PGP9.5, Uch-L1, UCHL-1, Neuron cytoplasmic protein 9.5
Gene Name
UCHL1
UniProt
P09936
Tag
Non-Tagged
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Neuroscience
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
24.8 kDa (223aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Contents
20mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA
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