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Human Dihydrolipoamide Dehydrogenase/DLD Recombinant Protein

Human Dihydrolipoamide Dehydrogenase/DLD Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 36-509aa. Application (s) : SDS-PAGE, Enzyme Activity.

Product Specifications

Synonyms

Dihydrolipoyl dehydrogenase mitochondrial, DLDH, E3, GCSL, LAD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, DLD, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, Glycine cleavage system L protein, Glycine cleavage system protein L, Lipoamide reductase, Lipoyl dehydrogenase, PHE 3

Gene Name

DLD

UniProt

P09622

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Metabolism

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 200 units/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

54.4 kDa (511aa)

Contents

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol

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