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Mouse Pgk1 Recombinant Protein

Mouse Pgk1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-417aa. Application (s) : Enzyme Activity, SDS-PAGE.

Product Specifications

Synonyms

Phosphoglycerate kinase 1, Pgk-1

Gene Name

Pgk1

UniProt

P09411

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Cardiovascular

Purification

> 90% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

47.1 kDa (441aa) confirmed by MALDI-TOF

Contents

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

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