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Mouse Alkaline Phosphatase/ALPL Recombinant Protein

Mouse Alkaline Phosphatase/ALPL Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 19-503aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS

Gene Name

Alpl

UniProt

P09242

Tag

His-Tag

Source

Baculovirus

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Cell Cycle

Purification

> 95% by SDS-PAGE

Concentration

0.5mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1 pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

54.5 kDa (493aa)

Contents

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

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