Human recombinant PROS1 protein, AF

PROS1, also known as protein S, is a vitamin K-dependent protein that plays a crucial role in the regulation of blood coagulation. It functions as a cofactor for protein C, another key player in the coagulation cascade. PROS1 acts as an anticoagulant by inhibiting the activity of certain clotting factors and promoting the breakdown of blood clots. It helps maintain the balance between clotting and anticoagulation, preventing the formation of excessive blood clots that can lead to thrombosis.

Product Specifications

Synonyms

PROS, Vitamin K-dependent protein S

Gene Name

PROS1

UniProt

P07225

Reactivity

Human

Tag

His-SUMO Tag (N-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>95% as determined by SDS-PAGE.

Endotoxin

<1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Testing in process

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 82.89 kDa. The protein migrates as 75 kDa uder reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.