Human Lactate Dehydrogenase B/LDHB Recombinant Protein
Human Lactate Dehydrogenase B/LDHB Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-334aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Renal carcinoma antigen NY-REN-46, Lactate dehydrogenase B, L-lactate dehydrogenase B chain, LDH-H, LDH-B, LDH heart subunit
Gene Name
LDHB
UniProt
P07195
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Cancer
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Observed Molecular Weight
38.8 kDa (354aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT
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