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Human Lactate Dehydrogenase B/LDHB Recombinant Protein

Human Lactate Dehydrogenase B/LDHB Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-334aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Renal carcinoma antigen NY-REN-46, Lactate dehydrogenase B, L-lactate dehydrogenase B chain, LDH-H, LDH-B, LDH heart subunit

Gene Name

LDHB

UniProt

P07195

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Cancer

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

38.8 kDa (354aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

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