Human Enolase 1/Non-neural enolase Recombinant Protein
Human Enolase 1/Non-neural enolase Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-434aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1, ENO1L1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, MBP1, MBPB1, MPB1, MYC promoter-binding protein 1, NNE, Non-Neuronal Enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH, Tau-crystallin
Gene Name
ENO1
UniProt
P06733
Tag
Non-Tagged
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Metabolism
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 20,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
47.1 kDa (434aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol
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