E.coli gor Recombinant Protein
E. coli gor Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-450aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Glutathione oxidoreductase, ECK3485, gorA, JW3467
Gene Name
Gor
UniProt
P06715
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Enzymes and Proteases
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 60 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37°C, pH 7.5.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
51.2 kDa (473aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
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