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E.coli gor Recombinant Protein

E. coli gor Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-450aa. Application (s) : Enzyme Activity, SDS-PAGE.

Product Specifications

Synonyms

Glutathione oxidoreductase, ECK3485, gorA, JW3467

Gene Name

Gor

UniProt

P06715

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Enzymes and Proteases

Purification

> 90% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 60 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37°C, pH 7.5.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

51.2 kDa (473aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

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