Human Alkaline Phosphatase/ALPP/ALPI Recombinant Protein
Human Alkaline Phosphatase/ALPP/ALPI Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 23-506aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
Gene Name
ALPP
UniProt
P05187
Tag
His-Tag
Source
Baculovirus
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
0.5mg/mL (determined by absorbance at 280nm)
Bioactivity
Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
53.9 kDa (494aa)
Contents
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
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