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Human Alkaline Phosphatase/ALPP/ALPI Recombinant Protein

Human Alkaline Phosphatase/ALPP/ALPI Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 23-506aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

Gene Name

ALPP

UniProt

P05187

Tag

His-Tag

Source

Baculovirus

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Enzymes and Proteases

Purification

> 95% by SDS-PAGE

Concentration

0.5mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

53.9 kDa (494aa)

Contents

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

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