Human GAPDH Recombinant Protein
Human GAPDH Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-335aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD
Gene Name
GAPDH
UniProt
P04406
Tag
Non-Tagged
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
36 kDa (335aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 1mM EDTA, 20% glycerol
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