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Human TNF-alpha Recombinant Protein

Human TNF-alpha Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 77-233aa. Application (s) : SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Gene Name

TNF

UniProt

P01375

Tag

His-Tag

Source

Baculovirus

Applications

SDS-PAGE, Bioactivity

Field of Research

Immunology

Purification

> 90% by SDS-PAGE

Concentration

1mg/mL (determined by absorbance at 280nm)

Bioactivity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/mL.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

18.1 kDa (163aa)

Contents

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

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