Human TNF-alpha Recombinant Protein
Human TNF-alpha Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 77-233aa. Application (s) : SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Gene Name
TNF
UniProt
P01375
Tag
His-Tag
Source
Baculovirus
Applications
SDS-PAGE, Bioactivity
Field of Research
Immunology
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by absorbance at 280nm)
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/mL.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
18.1 kDa (163aa)
Contents
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
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