Human recombinant IGF-II (Insulin-like growth factor-II) protein, AF

Insulin like Growth Factors 2 (IGF-II) is a 7.48 kDa member of the Insulin-like Growth Factors with 67 amino acid residues. IGF-II is mainly expressed from placenta, extravillous trophoblasts, leydig cells, syncytiotrophoblasts, cytotrophoblasts, peritubular cells. IGF-II regulating fetoplacental development and tissue differentiation. In adults, IGF-II signaling involves glucose metabolism in adipose tissue, skeletal muscle and liver. It also has important implications for metabolic disorders and cancer.

Product Specifications

Synonyms

Somatamedin A

Gene Name

IGF2

UniProt

P01344

Reactivity

Human

Tag

His Tag (N-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Measure by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is <3 ng/mL. The specific activity of recombinant human IGF-II is > 3x 105 IU/mg.

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 8.28 kDa. The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.