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Human Adenosine Deaminase/ADA Recombinant Protein

Human Adenosine Deaminase/ADA Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-363aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Adenosine deaminase, Adenosine aminohydrolase, ADA1

Gene Name

ADA

UniProt

P00813

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Enzymes and Proteases

Purification

> 85% by SDS-PAGE

Concentration

0.5mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is >40 units/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

42.9 kDa (383aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

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