Human Adenosine Deaminase/ADA Recombinant Protein
Human Adenosine Deaminase/ADA Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-363aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Adenosine deaminase, Adenosine aminohydrolase, ADA1
Gene Name
ADA
UniProt
P00813
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Enzymes and Proteases
Purification
> 85% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is >40 units/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
42.9 kDa (383aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
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