Mouse DHFR Recombinant Protein

Mouse DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Dihydrofolate reductase, EC 1.5.1.3

Gene Name

Dhfr

UniProt

P00375

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Redox Proteins

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)