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Human Dihydrofolate Reductase/DHFR Recombinant Protein

Human Dihydrofolate Reductase/DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application (s) : SDS-PAGE, Enzyme Activity.

Product Specifications

Synonyms

Dihydrofolate reductase, EC 1.5.1.3

Gene Name

DHFR

UniProt

P00374

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Redox Proteins

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

23.6 kDa (207aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol

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