Human Lactate Dehydrogenase A/LDHA Recombinant Protein
Human Lactate Dehydrogenase A/LDHA Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-332aa. Application (s) : SDS-PAGE, Enzyme Activity.
Product Specifications
Synonyms
Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59
Gene Name
LDHA
UniProt
P00338
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Redox Proteins
Purification
> 95% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
38.8 kDa (352aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
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