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Human recombinant IL-18BP (Interleukin-18-binding protein) protein, AF

IL18BP, specifically binds to IL-18, suppressing its activity. IL18BP lacks a transmembrane domain and is prominently expressed in the heart, lung, placenta, and spleen. Additionally, IL18BP regulates the early TH1 cytokine response by reducing IFN-gamma production, moderating T-helper type 1 immune responses.

Product Specifications

Synonyms

Tadekinig-alfa, IL18BP

Gene Name

IL18BP

UniProt

O95998

Reactivity

Human

Tag

His Tag (C-term)

Source

HEK293 cell

Applications

Cell Culture

Purification

>95% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Testing in process

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 18.44 kDa. The protein migrates as 35 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PROTO95998-4

AA Sequence

A DNA sequence encoding Human IL-18BP Protein (#O95998) (Met1-Gly194) was expressed with polyhistidine tag at the C-terminus.

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