Swine recombinant IL-17A (Interleukin-17A) protein, AF

Interleukin 17-alpha (IL-17A) is a 14.54 kDa cytokine with 131 amino acid residues and is also referred to as CTLA-8. IL-17A is a proinflammatory cytokine mainly expressed in Th 17 cells, NK cells, and innate lymphoid cells (ILCs) . After binding to the IL-17 receptor, IL-17A stimulates the production of IL-6, cyclooxygenase-2 (COX-2), and nitric oxide (NO) by activating NF-κB and MAPK signaling pathways. In addition, it also plays an essential role in against infection of extracellular bacteria and fungi by inducing epithelial cells to produce antimicrobial peptides.

Product Specifications

Synonyms

IL17A

Gene Name

IL17A

UniProt

B6E279

Reactivity

Swine

Tag

His Tag (C-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>98% as determined by SDS-PAGE.

Endotoxin

<0.01 EU per 1 μg of the protein by the LAL method.

Bioactivity

Testing in process

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 15.81 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

AA Sequence

MGIMIPQSPGCPKTEDKNFPQHVRVNLNILNRSTPARRPSDYSKRSTSPWTLQRNEDPERYPSVIWEAKCSHSGCINAEGKEDHHMNSVPIQQEILVLRREPRHCPNSFRLEKVMVTVGCTCVTPIVRHIS with polyhistidine tag at the C-terminus.